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DAVID CHUNG'S PAGE On December 9, 2003, I was visited by architect David Chung of Honolulu. We spent a great day together teaching each other and I shared with David the experiments that were going on. At that time, we were packing 15 Micro-Lobsters in our 1/2-gallon Introductory Breeder Tanks but I had trials going on with 24 Micro-Lobsters. He thought more was better, with more activity and volunteered to become a member of our Micro-Lobster Team to report from time to time. He handcarried a unit back to Honolulu with an extra dozen. So he began with 27 Micro-Lobsters in the 1/2-gallon tank. ~~~David W. Fukumoto (Fuku-Bonsai) _________________________________________________________ |
| THE SET-UP
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DECEMBER 10, 2003. I took the mini-breeder tank to work this morning and
set it up on my desk. The opae-ula are doing well and swimming around the container. Other
people nearby have commented very favorably about the setup, especially the fishermen, who
have made a couple of jokes about bait for fishing. Aloha, David
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| HIGHER POPULATION DENSITY CONCLUSION &
FEEDING RICE
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JANUARY 21, 2004. It's been approximately six weeks since I picked up the mini-breeder
tank. As you recall, my tank is the experiment to see what happens with a higher
population of opae-ula. They are constantly moving and swimming around. I can honestly say
that the tank is never without some kind of activity. The water is still clear and the
system shows no sign of distress, much less crashing. I would say that this experiment in
high-density living is a complete success! The opae-ula container is at my desk at work,
on a computer CPU next to the monitor and approximately 14 feet from a west-facing
full-height glass wall with semi-blackout curtains. I look at them all the time and it is
a wonderful relief from staring at a computer for extended periods. They are fed on a 3X
per week schedule on Mondays, Wednesdays, and Fridays. The air conditioning goes off after
5:00 PM and is off on the weekends. The opae-ula are certainly hungry creatures! They
sometimes get into "fisticuffs" jostling for the spirulina food. I see four
independently acting front "arms" with what looks like tiny claws. Fortunately,
with the size of these pinchers they cannot do much damage to each other. Foraging around
the tank, they normally touch noses when making contact or pretty much stay by themselves.
When I put a grain of raw rice into the water, they just glom on to it --- I have counted
up to 10 of them surrounding a grain of rice. Aloha, David
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| RAW RICE CLARIFICATION
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January 22,
2004 . Clarification: I add a single grain of RAW rice, sometimes two. It
attracts the shrimp right away, and soon there are 6-9 all trying to get at it. It takes
4-5 days to be consumed and when it gets to about half the size of a BB, it can be lifted
by a single opae-ula. Like eating a large watermelon, I guess. After a couple of hours,
though, the number on the grain of rice dwindles down to just a couple, and often just
one. The grain of rice is deposited to be near the side where it is clear of any gravel.
Aloha, David
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| RESOLVING AN ALGAE PROBLEM:
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April
4, 2004. Perhaps I have an over abundance of light at my work station, as the
walls of the mini-breeder tank are starting to get cloudy and the opae-ula are starting to
hang out more on the walls, parking themselves to eat the growth. The water is still
relatively clear although I have a thin film on the surface --- not oily, but some kind of
film. I am tempted to discontinue the spirulina feeding for a short while so the opae-ula
eat more of the algae, but I doubt that it will go away completely. I thought about
cleaning the walls with some 3M scotchbrite or nylon netting on a stick, but am concerned
about the disruption it would cause. What would you recommend? REPLY: Aloha David: Try cutting a bunch of Q-tips swabs and taping them to a chopstick in an overlapping pattern to scrub off the algae. Leave the residue in for a day or two and some of it will go to the surface. Use a turkey baster to suck off the film off the surface and removing 1/2" or so would be okay. Bring level up with distilled water. If necessary, repeat in a week or so. Stop feeding for a few weeks makes sense. ~~~David
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| SPOTTED FIRST BERRIED FEMALE!
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MAY 3, 2004 When I came to work today, I fed the shrimp, as they are on a M-W-F
feeding cycle. I had to look twice, but sure enough, one of the shrimp has eggs--didn't
expect this so soon! Are you getting any other reports of berried females this early in
the year? Aloha, David
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| REPORT ON LARVAE HATCHING!
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JUNE
4, 2004 (Friday) Here is a short memo on my observations: May 3, 2004: First sighting of a berried female and report to Fuku Bonsai. Told that incubation period was 38 days. Observed 6-7 eggs, with swimmerets seemingly fanning water over the area. June 2, 2004: Observed one of the eggs hanging below the others, trailing towards the rear of the egg clusters. June 3, 2004: 8:00 AM First baby shrimp! Little transparent body, mostly in the upside-down, or head down position. About 3/16" to 1/4" in length. The head area is a little darker, tapering towards the more transparent tail area. Seems to drift, but there is obviously no current in the tank. Will "popcorn" for about 1/2"-3/4" when in contact with other adult opae-ula. Changes direction of drift, so under some kind of power. With a magnifying glass, I can see movement in the area of the four front legs that in the adults have claws. June 3, 2004: 12:00 noon. Female shows about 4 eggs left. Female tries to shake off the babies, but they are coming off one at a time as they are ready. Can see eyes in the more "mature" babies. They are drifting all over the tank, up and down, left and right, and popcorning whenever they encounter the adults. The adults do not show any curiosity, and pretty much ignore them. Some of the babies have a light red color already. June 4, 2004: 8:00 AM. (Friday) All babies now separated from female. Count eight of them. A few are moving while in the horizontal position, but seemingly up-side down. The babies are so small! Some of the adults have grown to about 5/8" in length. My tank started with about 25-30 shrimp. The incubation period from first observation of berried female to hatching was 31 days. This is a virtual population explosion --- the number has increased by 25%! Aloha, David |
REPORT ON LARVAE AFTER THE FIRST WEEKEND
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JUNE 7, 2004 Monday. Great photo with the dime, and a very good sketch. Since my tank is on my desk at work, this is a 3-day report. All of the babies seemed to have survived the week-end, but I would not be surprised if there are some that expire, as the tiny creatures are hanging around the edges, and the adult shrimp are streaming around, sometimes colliding with the small offspring. It is like the freeway traffic in there, with the adults barreling around with abandon. There have been periods where 4-6 babies are drifting around the center of the tank near the outside wall. This is where they get run into often, say at least a couple of times per minute, sometimes more often. Now later in the day, the babies are pretty much clustered near the bottom, where there is hardly any cross traffic. It was as if someone told them: "Stop playing in the middle of the road, you are going to get run over". I've noticed that there are a couple of darker spots on the generally transparent tail section of the babies. If the tail section was a football field, the darker areas are on the 20 and 35 yard line. Aloha, David
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| REPORT ON ISOLATING THE LARVAE
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JUNE 14, 2004 Monday I got your materials on Friday, a state holiday, so it was only this morning that the babies were isolated. I did not use the net. In my home tank I had mosquito larvae, and the shrimp basically left them alone, and they were getting fairly large, so I bought a turkey baster and sucked them up and dumped them down the sink drain. The mosquito larvae are evasive but there was enough vacuum to get them into the baster. Sometimes I got only water, but usually it worked. I even got one juvenile opae-ula by accident, but squirted it back with no ill effects so far. I decided to use the same method with the baby shrimp, and it worked very well, and with minimal disturbance to the adults in the tank. This is the only way to do it, if you ask me. Relocation and isolation was done 11 days after "hatching". Once isolated, I counted nine (9) babies! I think isolation is a great idea, because they are now peacefully drifting around in the small plastic container, which is just the right footprint size. No adults to crash into them, and very little popcorning. In the tank with the adults, the babies were getting crashed into all the time. By and large, the babies are still in the head-down position. The babies sometimes run into each other, perhaps because they are usually moving backwards. Movement can be in any direction, but is usually backwards; head down, with the back inclined about 60 degrees from horizontal. With the loupe I am able to observe the legs moving and these may be the only means of locomotion. The swimmerets may be working, but if they are, it is too small to see. Perhaps development of the swimmerets is necessary for horizontal locomotion. I think isolation will be a big benefit to the baby shrimp. Aloha, David
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| OBSERVATIONS OF THE 2.5" PLASTIC BOX
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JUNE 15, 2004 Tuesday. From what I can see, your drawing seems fairly accurate.
After isolation, I was able to count ten (10) babies. When they get in the right focusing
distance from the loupe, I am able to see the legs moving. They remind me of baby Huey
Helicopters, soaring in different directions, usually backwards. Today, the 11th day since
hatching, I observed one baby starting to use its swimmerets and starting to swim in a
upright horizontal position, but just for a short while. I will have to wait and see if
there are more of them tomorrow with developed swimmerettes. Aloha, David
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| REPORT OF THE START OF LARVAE SWIMMING
HORIZONTALLY (START OF POST-LARVAL DEVELOPMENT STAGE)
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JUNE
16, 2004 Wednesday. Birth plus 13 days. Two of the babies are now swimming
horizontally. Correspondingly, these two show the swimmerets in action. So I am concluding
that the swimmerets need to be developed for horizontal motion to occur. Otherwise the
babies are basically helicopters with some ability to control direction, but they are
pretty clumsy. The rest of the babies are not very active, parked on the floor, walls, or
perched on the lava rock. Even when still, the front legs are constantly in motion. Some
are exhibiting jerky tail movement, like they are trying to free up the swimmerets in the
tail area.Aloha, David
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| NOTE: The initial "LARVAE" stage begins with the eggs hatching until they begin to swim horizontally. Thomas Iwai and Mike Yamamoto (DLNR) both observed multiple larvae 14-15 days in this stage. David observed it as 13-15 days. | JUNE
17, 2004 Thursday. 14th day, 4:00 PM: The babies all seem to be dormant at this
moment. If parked on the walls, the position seems to not matter --- up, down, sideways.
The single piece of rock is positioned on two points with an arch form between the points
of contact with the bottom, and a couple of shrimp are parked upside-down there. I would
guess that all but a couple of them are now able to swim horizontally. Aloha, David
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| NOTE: Initially researchers were questioning whether there were numerous moltings as the larvae developed as the tiny clear molt shells are extremely difficult to see.. David's observations were passed on to them to confirm molting as the larvae passes into the next stage. | JUNE
21, 2004 Monday (17th day): The babies all seem content to be parked and free of
any kind of movement. When the observation box is disturbed, they will swim about, but
arrive at a new location and park there. The babies are all swimming well and no
longer drifting about. At first, I was going to report that there was no evidence of molt
shells. Then I decided to disturb the water a bit and there they were floating around ---
so taransparent as to be nearly invisible! There seems to be at least three or four
molt shells--hard to tell because they have broken up somewhat. It appears that the
molting must have occurred in the 15th or 16th day. Some of the babies are coming up to to
surface, hopefully to feed on the small amount of food I placed there. Aloha, David
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| FIRST PHOTOS FROM DAVID CHUNG OF
POST-LARVAL STAGE NOTE: David used a digital camera with a 5x jeweler's loupe held in front to take the following photos using the technique discovered earlier by Bryan Yoshimura.
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JUNE 22, 2004 Tuesday (18th day) I took some digital photos this morning, and will try to send them tonight from my computer at home. I don't think the results are that great. The molt shells match the color of the acetate tank floor almost exactly, and I did not realize they were there until I agitated the water. Now after agitation I can see in some cases entire shells, from tail to antenna, floating around until it settles back to the floor of the tank. In retrospect I am sorry that I was not able to check for molt shells earlier. I have yet to see any in the process of shedding its shell. The babies are mostly parking themselves on the single piece of lava rock, and it has been difficult to confirm the total number left. I have confirmed at least eight, but am not certain whether ten still remain. Aloha, David |
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At this post-larval stage, they are about 2.6 millimeters or about 1/8" from
eye to tip of tail. David's apologizing for his less than perfect photography, but these
are amongst the best taken to date with ordinary cameras! When enlarged, the one on the top feeding upside down at the water line is clear while the other is out of focus. The depth of field is extremely small! |
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This is an ultra enlargement of the one that was feeding in the above photo #1. It was slightly enhanced and the image rotated so it appears right side up and may serve as a sketch guide for an attempted sketch. Thomas Iwai of Anuenue Fishery Research Center has taken outstanding photos through a dissecting microscope that show the details very clearly. Sketches were also part of a 1973 research paper published by Carl Couret, Jr. and Diana Wong that are reproduced in another part of this website with permission. |
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A second photo shows the tiny post-larvals taken through the 5x jeweler's loupe. The loupe
focuses about 2" away. So if the larvae are transfered into a 2.5" x 2.5" x
6" plastic box, it will be possible to view them in good detail. Use a
"turkey roaster-baster" to suck them out and transfer them. The one in the upper left is the sharpest of all and shown below. |
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This is the best photo that I've received to date of a post-larvae opae-ula and will be making a sketch based upon it. The efforts to sketch combine the details possible from dissecting microscope photos with the posing that is only possible from actual photos. This was the only image which shows the relative size of the eyes. |
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A cropped portion of a greatly enlarged section of David's photo #6. The round design is plastic extrusion mark which measures about 8mm (a little more than 1/4"). The body length is less than 1/3 of the 8mm or about 2.66mm. |
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This is another cropped portion of a greatly enlarged section of David's photo #7 that has several larvae. Although fairly close together, only one is in better focus. Photographing such tiny creatures is a difficult challenge. I was able to obtain the fairly clear photograph of the first stage larvae shown at the start of this page with a 5x jeweler's loupe attached to a skylight filter on a 35mm single lens reflex camera mounted on a tripod. We welcome additional photos! |
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Based upon the reports of David Chung,
Bryan Yoshimura, others, and our own observations, the "post-larvae" sketch at
left and the following blurb created as part of Micro-Lobster Reproduction Cycle Summary. 4. POST-LARVAE. Once into the postlarval stage, swimmerets are sufficiently developed to allow swimming horizontally. Up to this stage, a yolk sack sustained them. Now they begin feedin. The tend to hide amongst the rocks a lot. After a while they begin to feel secure and venture out more often. Actual body length has not changed much. The head is proportionally large and the tail still undeveloped. In a few weeks all appendages develop, they enter the "juvenile" stage where they appear as miniature replicas of adults and this is an increase in the growth rate. |
CONCLUSION TO PART I: Opae-ula was only scientifically identified by L. B. Holthius in 1963 and there are very few significant published papers. We are fortunate to be in contact with Dr. Richard Brock and Thomas Iwai who at this point must be considered the leading authorities on opae-ula. It is possible that there are others who may know more, but for their own reasons, will not share their knowledge. Fuku-Bonsai is committed to publicly research and invites everyone to send their detailed observations and photographs. Join our Micro-Lobster Team.
Fuku-Bonsai introduced it's first Micro-Lobster 1/2-gallon Aquarium one year ago and it has already been upgrades several times and is now known as the 1/2-gallon Introductory Mini Breeder Tank. There have been a number of reports of berried females, eggs hatching, and larval developing into juveniles and adults. Bryan Yoshimura's earlier report was based upon 5 eggs hatching and all 5 successfully maturing into adults.
David Chung's report is the first day-by-day report and I thank him for sharing it with everyone. Having ten larvae is believed to be amongst the highest with such a detailed record. Generally, eggs that are aborted before four weeks are not likely to hatch. David's full unedited email compilations are being sent to the researchers to aid them with comparative observations. At times, the researchers are able to point out what is likely to happen next and it's a win-win benefit. I look forward to Thomas Iwai publishing his research paper and continuing into David Chung's Part II. MAHALO DAVID!
~~~David W. Fukumoto, Fuku-Bonsai
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- DAVID CHUNG REPORTS A RECORD 24 LARVAE HATCHED AND ALL SURVIVED!
- *** Go to David Chung's Page 2!
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